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Titel: | Use of antibodies against Epstein-Barr virus nuclear antigen 1 for detection of cellular proteins with monomethylated arginine residues that are potentially involved in viral transformation |
VerfasserIn: | Graesser, Christian Nord, Ruth Flaswinkel, Heinrich Kremmer, Elisabeth Meese, Eckart Caban, Karolina Magdalena Fröhlich, Thomas Grässer, Friedrich A. Hart, Martin |
Sprache: | Englisch |
Titel: | Archives of Virology |
Bandnummer: | 169 |
Heft: | 12 |
Verlag/Plattform: | Springer Nature |
Erscheinungsjahr: | 2024 |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Epstein–Barr virus nuclear antigen 1 (EBNA1) contains two arginine-glycine (RG) repeats that contain symmetric/asymmetric dimethylarginine (SDMA/ADMA) and monomethylarginine (MMA) residues. We generated mouse monoclonal antibodies directed against a monomethylated GRGRGG-containing repeat located between amino acids 328 and 377 of EBNA1. In addition to detecting MMA-modified EBNA1, we also had the goal of identifying cellular proteins that bind to MMA-modified EBNA1 in EBV-positive Raji cells. Furthermore, we hypothesized that antibodies against MMA-modified EBNA1 might also recognize cell factors that use an MMA-modified surface structure similar to that of EBNA1 to bind to their common targets. Using a combination of immunoprecipitation and mass spectrometry, we identified a number of such cellular proteins, including SNRPD1-3, ALY/REF, RPS15, DIDO1, LSM12, LSM14A, DAP3, and CPSF1. An NACA complex protein that was shown previously to bind to the glycine-alanine repeat of EBNA1 was also identified. The proteins identified in this study are involved in splicing, tumorigenesis, transcriptional activation, DNA stability, and RNA processing or export. |
DOI der Erstveröffentlichung: | 10.1007/s00705-024-06172-7 |
URL der Erstveröffentlichung: | https://link.springer.com/article/10.1007/s00705-024-06172-7 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-436049 hdl:20.500.11880/39064 |
ISSN: | 1432-8798 0304-8608 |
Datum des Eintrags: | 29-Nov-2024 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Information |
In Beziehung stehendes Objekt: | https://static-content.springer.com/esm/art%3A10.1007%2Fs00705-024-06172-7/MediaObjects/705_2024_6172_MOESM1_ESM.tif https://static-content.springer.com/esm/art%3A10.1007%2Fs00705-024-06172-7/MediaObjects/705_2024_6172_MOESM2_ESM.tif https://static-content.springer.com/esm/art%3A10.1007%2Fs00705-024-06172-7/MediaObjects/705_2024_6172_MOESM3_ESM.xlsx https://static-content.springer.com/esm/art%3A10.1007%2Fs00705-024-06172-7/MediaObjects/705_2024_6172_MOESM4_ESM.docx |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Humangenetik M - Infektionsmedizin |
Professur: | M - Prof. Dr. Eckart Meese M - Keiner Professur zugeordnet |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Größe | Format | |
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s00705-024-06172-7.pdf | 1,43 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons