Please use this identifier to cite or link to this item:
doi:10.22028/D291-34888
Title: | A Promiscuous Halogenase for the Derivatization of Flavonoids |
Author(s): | Kolling, Dominik Stierhof, Marc Lasch, Constanze Myronovskyi, Maksym Luzhetskyy, Andriy |
Language: | English |
Title: | Molecules |
Volume: | 26 |
Issue: | 20 |
Publisher/Platform: | MDPI |
Year of Publication: | 2021 |
Free key words: | halogenase flavonoid heterologous expression Frankia alni Streptomyces albus Del14 |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | Halogenation often improves the bioactive properties of natural products and is used in pharmaceutical research for the generation of new potential drug leads. High regio- and stereospecificity, simple reaction conditions and straightforward downstream processing are the main advantages of halogenation using enzymatic biocatalysts compared to chemical synthetic approaches. The identification of new promiscuous halogenases for the modification of various natural products is of great interest in modern drug discovery. In this paper, we report the identification of a new promiscuous FAD-dependent halogenase, DklH, from Frankia alni ACN14a. The identified halogenase readily modifies various flavonoid compounds, including those with well-studied biological activities. This halogenase has been demonstrated to modify not only flavones and isoflavones, but also flavonols, flavanones and flavanonols. The structural requirements for DklH substrate recognition were determined using a feeding approach. The homology model of DklH and the mechanism of substrate recognition are also proposed in this paper. |
DOI of the first publication: | 10.3390/molecules26206220 |
Link to this record: | urn:nbn:de:bsz:291--ds-348888 hdl:20.500.11880/31917 http://dx.doi.org/10.22028/D291-34888 |
ISSN: | 1420-3049 |
Date of registration: | 27-Oct-2021 |
Description of the related object: | Supplementary Material |
Related object: | https://www.mdpi.com/1420-3049/26/20/6220/s1 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Professorship: | NT - Prof. Dr. Andriy Luzhetskyy |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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File | Description | Size | Format | |
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molecules-26-06220.pdf | 2,38 MB | Adobe PDF | View/Open |
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