Please use this identifier to cite or link to this item: doi:10.22028/D291-34888
Title: A Promiscuous Halogenase for the Derivatization of Flavonoids
Author(s): Kolling, Dominik
Stierhof, Marc
Lasch, Constanze
Myronovskyi, Maksym
Luzhetskyy, Andriy
Language: English
Title: Molecules
Volume: 26
Issue: 20
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: halogenase
flavonoid
heterologous expression
Frankia alni
Streptomyces albus Del14
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Halogenation often improves the bioactive properties of natural products and is used in pharmaceutical research for the generation of new potential drug leads. High regio- and stereospecificity, simple reaction conditions and straightforward downstream processing are the main advantages of halogenation using enzymatic biocatalysts compared to chemical synthetic approaches. The identification of new promiscuous halogenases for the modification of various natural products is of great interest in modern drug discovery. In this paper, we report the identification of a new promiscuous FAD-dependent halogenase, DklH, from Frankia alni ACN14a. The identified halogenase readily modifies various flavonoid compounds, including those with well-studied biological activities. This halogenase has been demonstrated to modify not only flavones and isoflavones, but also flavonols, flavanones and flavanonols. The structural requirements for DklH substrate recognition were determined using a feeding approach. The homology model of DklH and the mechanism of substrate recognition are also proposed in this paper.
DOI of the first publication: 10.3390/molecules26206220
Link to this record: urn:nbn:de:bsz:291--ds-348888
hdl:20.500.11880/31917
http://dx.doi.org/10.22028/D291-34888
ISSN: 1420-3049
Date of registration: 27-Oct-2021
Description of the related object: Supplementary Material
Related object: https://www.mdpi.com/1420-3049/26/20/6220/s1
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Andriy Luzhetskyy
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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