Please use this identifier to cite or link to this item: doi:10.22028/D291-34829
Title: Organ-Specific Uptake of Extracellular Vesicles Secreted by Urological Cancer Cells
Author(s): Linxweiler, Johannes
Kolbinger, Anja
Himbert, Dirk
Zeuschner, Philip
Saar, Matthias
Stöckle, Michael
Junker, Kerstin
Language: English
Title: Cancers
Volume: 13
Issue: 19
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: prostate cancer
bladder cancer
kidney cancer
extracellular vesicles
organotropism
organ-specific
premetastatic niche
intercellular communication
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Extracellular vesicles (EVs) secreted by cancer cells have been shown to take a pivotal part in the process of local and systemic tumor progression by promoting the formation of a supportive local tumor microenvironment and preparing premetastatic niches in distant organ systems. In this study, we analyzed the organ-specific uptake of EVs secreted by urological cancer cells using an innovative in-vivo approach. EVs from benign and malignant prostate, kidney, and bladder cells were isolated using ultracentrifugation, fluorescence-labeled and injected intravenously in immunodeficient mice. After 12 or 24 h, the animals were sacrificed, their organs were harvested and analyzed for the presence of EVs by high-resolution fluorescence microscopy. Across all entities, EVs were taken up fast (12 h > 24 h), and EVs from malignant cells were taken up more efficiently than EVs from benign cells. Though not entirely organ-specific, EVs were incorporated in different amounts, depending on the entity (prostate: lung > liver > brain; kidney: brain > lung > liver; bladder: lung > liver > brain). EV uptake in other organs than lung, liver, brain, and spleen was not observed. Our results suggest a role of EVs in the formation of premetastatic niches and an organotropism in EV uptake, which have to be examined in more detail in further studies.
DOI of the first publication: 10.3390/cancers13194937
Link to this record: urn:nbn:de:bsz:291--ds-348299
hdl:20.500.11880/31842
http://dx.doi.org/10.22028/D291-34829
ISSN: 2072-6694
Date of registration: 13-Oct-2021
Description of the related object: Supplementary Material
Related object: https://www.mdpi.com/2072-6694/13/19/4937/s1
Faculty: M - Medizinische Fakultät
Department: M - Urologie und Kinderurologie
Professorship: M - Prof. Dr. Michael Stöckle
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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