Please use this identifier to cite or link to this item:
doi:10.22028/D291-34829
Title: | Organ-Specific Uptake of Extracellular Vesicles Secreted by Urological Cancer Cells |
Author(s): | Linxweiler, Johannes Kolbinger, Anja Himbert, Dirk Zeuschner, Philip Saar, Matthias Stöckle, Michael Junker, Kerstin |
Language: | English |
Title: | Cancers |
Volume: | 13 |
Issue: | 19 |
Publisher/Platform: | MDPI |
Year of Publication: | 2021 |
Free key words: | prostate cancer bladder cancer kidney cancer extracellular vesicles organotropism organ-specific premetastatic niche intercellular communication |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Extracellular vesicles (EVs) secreted by cancer cells have been shown to take a pivotal part in the process of local and systemic tumor progression by promoting the formation of a supportive local tumor microenvironment and preparing premetastatic niches in distant organ systems. In this study, we analyzed the organ-specific uptake of EVs secreted by urological cancer cells using an innovative in-vivo approach. EVs from benign and malignant prostate, kidney, and bladder cells were isolated using ultracentrifugation, fluorescence-labeled and injected intravenously in immunodeficient mice. After 12 or 24 h, the animals were sacrificed, their organs were harvested and analyzed for the presence of EVs by high-resolution fluorescence microscopy. Across all entities, EVs were taken up fast (12 h > 24 h), and EVs from malignant cells were taken up more efficiently than EVs from benign cells. Though not entirely organ-specific, EVs were incorporated in different amounts, depending on the entity (prostate: lung > liver > brain; kidney: brain > lung > liver; bladder: lung > liver > brain). EV uptake in other organs than lung, liver, brain, and spleen was not observed. Our results suggest a role of EVs in the formation of premetastatic niches and an organotropism in EV uptake, which have to be examined in more detail in further studies. |
DOI of the first publication: | 10.3390/cancers13194937 |
Link to this record: | urn:nbn:de:bsz:291--ds-348299 hdl:20.500.11880/31842 http://dx.doi.org/10.22028/D291-34829 |
ISSN: | 2072-6694 |
Date of registration: | 13-Oct-2021 |
Description of the related object: | Supplementary Material |
Related object: | https://www.mdpi.com/2072-6694/13/19/4937/s1 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Urologie und Kinderurologie |
Professorship: | M - Prof. Dr. Michael Stöckle |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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cancers-13-04937-v2.pdf | 5,69 MB | Adobe PDF | View/Open |
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