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Titel: Organ-Specific Uptake of Extracellular Vesicles Secreted by Urological Cancer Cells
VerfasserIn: Linxweiler, Johannes
Kolbinger, Anja
Himbert, Dirk
Zeuschner, Philip
Saar, Matthias
Stöckle, Michael
Junker, Kerstin
Sprache: Englisch
Titel: Cancers
Bandnummer: 13
Heft: 19
Verlag/Plattform: MDPI
Erscheinungsjahr: 2021
Freie Schlagwörter: prostate cancer
bladder cancer
kidney cancer
extracellular vesicles
organotropism
organ-specific
premetastatic niche
intercellular communication
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Extracellular vesicles (EVs) secreted by cancer cells have been shown to take a pivotal part in the process of local and systemic tumor progression by promoting the formation of a supportive local tumor microenvironment and preparing premetastatic niches in distant organ systems. In this study, we analyzed the organ-specific uptake of EVs secreted by urological cancer cells using an innovative in-vivo approach. EVs from benign and malignant prostate, kidney, and bladder cells were isolated using ultracentrifugation, fluorescence-labeled and injected intravenously in immunodeficient mice. After 12 or 24 h, the animals were sacrificed, their organs were harvested and analyzed for the presence of EVs by high-resolution fluorescence microscopy. Across all entities, EVs were taken up fast (12 h > 24 h), and EVs from malignant cells were taken up more efficiently than EVs from benign cells. Though not entirely organ-specific, EVs were incorporated in different amounts, depending on the entity (prostate: lung > liver > brain; kidney: brain > lung > liver; bladder: lung > liver > brain). EV uptake in other organs than lung, liver, brain, and spleen was not observed. Our results suggest a role of EVs in the formation of premetastatic niches and an organotropism in EV uptake, which have to be examined in more detail in further studies.
DOI der Erstveröffentlichung: 10.3390/cancers13194937
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-348299
hdl:20.500.11880/31842
http://dx.doi.org/10.22028/D291-34829
ISSN: 2072-6694
Datum des Eintrags: 13-Okt-2021
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Material
In Beziehung stehendes Objekt: https://www.mdpi.com/2072-6694/13/19/4937/s1
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Urologie und Kinderurologie
Professur: M - Prof. Dr. Michael Stöckle
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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