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doi:10.22028/D291-34829
Titel: | Organ-Specific Uptake of Extracellular Vesicles Secreted by Urological Cancer Cells |
VerfasserIn: | Linxweiler, Johannes Kolbinger, Anja Himbert, Dirk Zeuschner, Philip Saar, Matthias Stöckle, Michael Junker, Kerstin |
Sprache: | Englisch |
Titel: | Cancers |
Bandnummer: | 13 |
Heft: | 19 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2021 |
Freie Schlagwörter: | prostate cancer bladder cancer kidney cancer extracellular vesicles organotropism organ-specific premetastatic niche intercellular communication |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Extracellular vesicles (EVs) secreted by cancer cells have been shown to take a pivotal part in the process of local and systemic tumor progression by promoting the formation of a supportive local tumor microenvironment and preparing premetastatic niches in distant organ systems. In this study, we analyzed the organ-specific uptake of EVs secreted by urological cancer cells using an innovative in-vivo approach. EVs from benign and malignant prostate, kidney, and bladder cells were isolated using ultracentrifugation, fluorescence-labeled and injected intravenously in immunodeficient mice. After 12 or 24 h, the animals were sacrificed, their organs were harvested and analyzed for the presence of EVs by high-resolution fluorescence microscopy. Across all entities, EVs were taken up fast (12 h > 24 h), and EVs from malignant cells were taken up more efficiently than EVs from benign cells. Though not entirely organ-specific, EVs were incorporated in different amounts, depending on the entity (prostate: lung > liver > brain; kidney: brain > lung > liver; bladder: lung > liver > brain). EV uptake in other organs than lung, liver, brain, and spleen was not observed. Our results suggest a role of EVs in the formation of premetastatic niches and an organotropism in EV uptake, which have to be examined in more detail in further studies. |
DOI der Erstveröffentlichung: | 10.3390/cancers13194937 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-348299 hdl:20.500.11880/31842 http://dx.doi.org/10.22028/D291-34829 |
ISSN: | 2072-6694 |
Datum des Eintrags: | 13-Okt-2021 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Material |
In Beziehung stehendes Objekt: | https://www.mdpi.com/2072-6694/13/19/4937/s1 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Urologie und Kinderurologie |
Professur: | M - Prof. Dr. Michael Stöckle |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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cancers-13-04937-v2.pdf | 5,69 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons