Please use this identifier to cite or link to this item:
doi:10.22028/D291-34584
Title: | Targeted Lipidomics of Mitochondria in a Cellular Alzheimer’s Disease Model |
Author(s): | Kurokin, Irina Lauer, Anna Andrea Janitschke, Daniel Winkler, Jakob Theiss, Elena Leoni Griebsch, Lea Victoria Pilz, Sabrina Melanie Matschke, Veronika van der Laan, Martin Grimm, Heike Sabine Hartmann, Tobias Grimm, Marcus Otto Walter |
Language: | English |
Title: | Biomedicines |
Volume: | 9 |
Issue: | 8 |
Publisher/Platform: | MDPI |
Year of Publication: | 2021 |
Free key words: | mitochondria Alzheimer’s disease neurodegeneration lipidomics unsaturated fatty acids phosphatidylcholine phosphatidylethanolamine lyso-phospholipids plasmalogens carnitine carrier system |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Alzheimer’s disease (AD) is neuropathologically characterized by the accumulation of Amyloid-β (Aβ) in senile plaques derived from amyloidogenic processing of a precursor protein (APP). Recently, changes in mitochondrial function have become in the focus of the disease. Whereas a link between AD and lipid-homeostasis exists, little is known about potential alterations in the lipid composition of mitochondria. Here, we investigate potential changes in the main mitochondrial phospholipid classes phosphatidylcholine, phosphatidylethanolamine and the corresponding plasmalogens and lyso-phospholipids of a cellular AD-model (SH-SY5Y APPswedish transfected cells), comparing these results with changes in cell-homogenates. Targeted shotgun-lipidomics revealed lipid alterations to be specific for mitochondria and cannot be predicted from total cell analysis. In particular, lipids containing three and four times unsaturated fatty acids (FA X:4), such as arachidonic-acid, are increased, whereas FA X:6 or X:5, such as eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), are decreased. Additionally, PE plasmalogens are increased in contrast to homogenates. Results were confirmed in another cellular AD model, having a lower affinity to amyloidogenic APP processing. Besides several similarities, differences in particular in PE species exist, demonstrating that differences in APP processing might lead to specific changes in lipid homeostasis in mitochondria. Importantly, the observed lipid alterations are accompanied by changes in the carnitine carrier system, also suggesting an altered mitochondrial functionality |
DOI of the first publication: | 10.3390/biomedicines9081062 |
Link to this record: | urn:nbn:de:bsz:291--ds-345847 hdl:20.500.11880/31657 http://dx.doi.org/10.22028/D291-34584 |
ISSN: | 2227-9059 |
Date of registration: | 27-Aug-2021 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/article/10.3390/biomedicines9081062/s1 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Medizinische Biochemie und Molekularbiologie M - Neurologie und Psychiatrie |
Professorship: | M - Prof. Dr. Tobias Hartmann M - Prof. Dr. Martin Van der Laan |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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biomedicines-09-01062-v2.pdf | 6,12 MB | Adobe PDF | View/Open |
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