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Titel: Targeted Lipidomics of Mitochondria in a Cellular Alzheimer’s Disease Model
VerfasserIn: Kurokin, Irina
Lauer, Anna Andrea
Janitschke, Daniel
Winkler, Jakob
Theiss, Elena Leoni
Griebsch, Lea Victoria
Pilz, Sabrina Melanie
Matschke, Veronika
van der Laan, Martin
Grimm, Heike Sabine
Hartmann, Tobias
Grimm, Marcus Otto Walter
Sprache: Englisch
Titel: Biomedicines
Bandnummer: 9
Heft: 8
Verlag/Plattform: MDPI
Erscheinungsjahr: 2021
Freie Schlagwörter: mitochondria
Alzheimer’s disease
neurodegeneration
lipidomics
unsaturated fatty acids
phosphatidylcholine
phosphatidylethanolamine
lyso-phospholipids
plasmalogens
carnitine carrier system
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Alzheimer’s disease (AD) is neuropathologically characterized by the accumulation of Amyloid-β (Aβ) in senile plaques derived from amyloidogenic processing of a precursor protein (APP). Recently, changes in mitochondrial function have become in the focus of the disease. Whereas a link between AD and lipid-homeostasis exists, little is known about potential alterations in the lipid composition of mitochondria. Here, we investigate potential changes in the main mitochondrial phospholipid classes phosphatidylcholine, phosphatidylethanolamine and the corresponding plasmalogens and lyso-phospholipids of a cellular AD-model (SH-SY5Y APPswedish transfected cells), comparing these results with changes in cell-homogenates. Targeted shotgun-lipidomics revealed lipid alterations to be specific for mitochondria and cannot be predicted from total cell analysis. In particular, lipids containing three and four times unsaturated fatty acids (FA X:4), such as arachidonic-acid, are increased, whereas FA X:6 or X:5, such as eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), are decreased. Additionally, PE plasmalogens are increased in contrast to homogenates. Results were confirmed in another cellular AD model, having a lower affinity to amyloidogenic APP processing. Besides several similarities, differences in particular in PE species exist, demonstrating that differences in APP processing might lead to specific changes in lipid homeostasis in mitochondria. Importantly, the observed lipid alterations are accompanied by changes in the carnitine carrier system, also suggesting an altered mitochondrial functionality
DOI der Erstveröffentlichung: 10.3390/biomedicines9081062
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-345847
hdl:20.500.11880/31657
http://dx.doi.org/10.22028/D291-34584
ISSN: 2227-9059
Datum des Eintrags: 27-Aug-2021
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Materials
In Beziehung stehendes Objekt: https://www.mdpi.com/article/10.3390/biomedicines9081062/s1
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Medizinische Biochemie und Molekularbiologie
M - Neurologie und Psychiatrie
Professur: M - Prof. Dr. Tobias Hartmann
M - Prof. Dr. Martin Van der Laan
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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