Please use this identifier to cite or link to this item: doi:10.22028/D291-34456
Title: Discovery and Heterologous Production of New Cyclic Depsibosamycins
Author(s): Stierhof, Marc
Myronovskyi, Maksym
Zapp, Josef
Luzhetskyy, Andriy
Language: English
Title: Microorganisms
Volume: 9
Issue: 7
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: bosamycin
Streptomyces
heterologous expression
NRPS
cyclic peptide
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Streptomyces are producers of valuable secondary metabolites with unique scaffolds that perform a plethora of biological functions. Nonribosomal peptides are of special interest due to their variety and complexity. They are synthesized by nonribosomal peptide synthetases, large biosynthetic machineries that are encoded in the genome of many Streptomyces species. The identification of new peptides and the corresponding biosynthetic gene clusters is of major interest since knowledge can be used to facilitate combinatorial biosynthesis and chemical semisynthesis of natural products. The recently discovered bosamycins are linear octapeptides with an interesting 5-OMe tyrosine moiety and various modifications at the N-terminus. In this study, the new cyclic depsibosamycins B, C, and D from Streptomyces aurantiacus LU19075 were discovered. In comparison to the linear bosamycins B, C, and D, which were also produced by the strain, the cyclic depsibosamycins showed a side-chain-to-tail lactonization of serine and glycine, leading to a ring of four amino acids. In silico identification and heterologous expression of the depsibosamycin (dbm) gene cluster indicated that the cyclic peptides, rather than the linear derivatives, are the main products of the cluster.
DOI of the first publication: 10.3390/microorganisms9071396
Link to this record: urn:nbn:de:bsz:291--ds-344560
hdl:20.500.11880/31605
http://dx.doi.org/10.22028/D291-34456
ISSN: 2076-2607
Date of registration: 3-Aug-2021
Description of the related object: Supplementary Materials
Related object: https://www.mdpi.com/article/10.3390/microorganisms9071396/s1
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Alexandra K. Kiemer
NT - Prof. Dr. Andriy Luzhetskyy
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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