Please use this identifier to cite or link to this item:
doi:10.22028/D291-34456
Title: | Discovery and Heterologous Production of New Cyclic Depsibosamycins |
Author(s): | Stierhof, Marc Myronovskyi, Maksym Zapp, Josef Luzhetskyy, Andriy |
Language: | English |
Title: | Microorganisms |
Volume: | 9 |
Issue: | 7 |
Publisher/Platform: | MDPI |
Year of Publication: | 2021 |
Free key words: | bosamycin Streptomyces heterologous expression NRPS cyclic peptide |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | Streptomyces are producers of valuable secondary metabolites with unique scaffolds that perform a plethora of biological functions. Nonribosomal peptides are of special interest due to their variety and complexity. They are synthesized by nonribosomal peptide synthetases, large biosynthetic machineries that are encoded in the genome of many Streptomyces species. The identification of new peptides and the corresponding biosynthetic gene clusters is of major interest since knowledge can be used to facilitate combinatorial biosynthesis and chemical semisynthesis of natural products. The recently discovered bosamycins are linear octapeptides with an interesting 5-OMe tyrosine moiety and various modifications at the N-terminus. In this study, the new cyclic depsibosamycins B, C, and D from Streptomyces aurantiacus LU19075 were discovered. In comparison to the linear bosamycins B, C, and D, which were also produced by the strain, the cyclic depsibosamycins showed a side-chain-to-tail lactonization of serine and glycine, leading to a ring of four amino acids. In silico identification and heterologous expression of the depsibosamycin (dbm) gene cluster indicated that the cyclic peptides, rather than the linear derivatives, are the main products of the cluster. |
DOI of the first publication: | 10.3390/microorganisms9071396 |
Link to this record: | urn:nbn:de:bsz:291--ds-344560 hdl:20.500.11880/31605 http://dx.doi.org/10.22028/D291-34456 |
ISSN: | 2076-2607 |
Date of registration: | 3-Aug-2021 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/article/10.3390/microorganisms9071396/s1 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Professorship: | NT - Prof. Dr. Alexandra K. Kiemer NT - Prof. Dr. Andriy Luzhetskyy |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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microorganisms-09-01396.pdf | 1,85 MB | Adobe PDF | View/Open |
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