Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
doi:10.22028/D291-34092
Titel: | Dysregulated levels of glycogen synthase kinase-3β (GSK-3β) and miR-135 in peripheral blood samples of cases with nephrotic syndrome |
VerfasserIn: | Ardalan, Mohammadreza Hejazian, Seyyedeh Mina Sharabiyani, Hassan Fazlazar Farnood, Farahnoosh Ghafari Aghdam, Amirhossein Bastami, Milad Ahmadian, Elham Zununi Vahed, Sepideh Cucchiarini, Magali |
Sprache: | Englisch |
Titel: | PeerJ |
Bandnummer: | 8 |
Verlag/Plattform: | PeerJ |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | Nephrotic syndrome Proteinuria Focal Segmental Glomerulosclerosis Membranous glomerulonephritis miR-135a-5p GSK-3β |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Background. Glycogen synthase kinase-3 (GSK-3β) is a serine/threonine kinase with multifunctions in various physiological procedures. Aberrant level of GSK-3β in kidney cells has a harmful role in podocyte injury. Methods. In this article, the expression levels of GSK-3β and one of its upstream regulators, miR-135a-5p, were measured in peripheral blood mononuclear cells (PBMCs) of cases with the most common types of nephrotic syndrome (NS); focal segmental glomerulosclerosis (FSGS) and membranous glomerulonephritis (MGN). In so doing, fifty-two cases along with twenty-four healthy controls were included based on the strict criteria. Results. Levels of GSK-3β mRNA and miR-135 were measured with quantitative realtime PCR. There were statistically significant increases in GSK-3β expression level in NS (P = 0.001), MGN (P = 0.002), and FSGS (P = 0.015) groups compared to the control group. Dysregulated levels of miR-135a-5p in PBMCs was not significant between the studied groups. Moreover, a significant decrease was observed in the expression level of miR-135a-5p in the plasma of patients with NS (P = 0.020), MGN (P = 0.040), and FSGS (P = 0.046) compared to the control group. ROC curve analysis approved a diagnostic power of GSK-3β in discriminating patients from healthy controls (AUC: 0.72, P = 0.002) with high sensitivity and specificity. Conclusions. Dysregulated levels of GSK-3β and its regulator miR-135a may participate in the pathogenesis of NS with different etiology. Therefore, more research is needed for understanding the relationship between them. |
DOI der Erstveröffentlichung: | 10.7717/peerj.10377 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-340928 hdl:20.500.11880/31352 http://dx.doi.org/10.22028/D291-34092 |
ISSN: | 2167-8359 |
Datum des Eintrags: | 21-Mai-2021 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplemental Information |
In Beziehung stehendes Objekt: | https://dfzljdn9uc3pi.cloudfront.net/2020/10377/1/ROC-miR-135_Figure_S1.pdf https://dfzljdn9uc3pi.cloudfront.net/2020/10377/1/PBMC_gene_expression-row_data.xlsx |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Orthopädie |
Professur: | M - Prof. Dr. Henning Madry |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
peerj-10377.pdf | 1,08 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons