Please use this identifier to cite or link to this item: doi:10.22028/D291-31954
Title: Itaconic Acid Increases the Efficacy of Tobramycin against Pseudomonas aeruginosa Biofilms
Author(s): Ho, Duy-Khiet
De Rossi, Chiara
Loretz, Brigitta
Murgia, Xabier
Lehr, Claus-Michael
Language: English
Title: Pharmaceutics
Volume: 12
Issue: 8
Publisher/Platform: MDPI
Year of Publication: 2020
Free key words: tobramycin
ciprofloxacin
itaconic acid
itaconate
Pseudomonas aeruginosa
biofilms
infections
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: The search for novel therapeutics against pulmonary infections, in particular Pseudomonas aeruginosa (PA) biofilm infections, has been intense to deal with the emergent rise of antimicrobial resistance. Despite the numerous achievements in drug discovery and delivery strategies, only a limited number of therapeutics reach the clinic. To allow a timely preclinical development, a formulation should be highly effective, safe, and most importantly facile to produce. Thus, a simple combination of known actives that enhances the therapeutic efficacy would be a preferential choice compared to advanced drug delivery systems. In this study, we propose a novel combination of an anti-inflammatory agent—itaconic acid (itaconate, IA)—and an approved antibiotic—tobramycin (Tob) or ciprofloxacin (Cipro). The combination of Tob and IA at a molar ratio of 1:5 increased the biofilm eradicating efficacy in the strain PA14 wild type (wt) by ~4-fold compared to Tob alone. In contrast, such effect was not observed for the combination of IA with Cipro. Subsequent studies on the influence of IA on bacterial growth, pyocyanin production, and Tob biofilm penetration indicated that complexation with IA enhanced the transport of Tob through the biofilm. We recommend the simple and effective combination of Tob:IA for further testing in advanced preclinical models of PA biofilm infections.
DOI of the first publication: 10.3390/pharmaceutics12080691
Link to this record: urn:nbn:de:bsz:291--ds-319543
hdl:20.500.11880/30358
http://dx.doi.org/10.22028/D291-31954
ISSN: 1999-4923
Date of registration: 11-Jan-2021
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Claus-Michael Lehr
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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