Please use this identifier to cite or link to this item: doi:10.22028/D291-31352
Title: Protein Kinase CK2 Regulates Nerve/Glial Antigen (NG)2-Mediated Angiogenic Activity of Human Pericytes
Author(s): Schmitt, Beate M.
Boewe, Anne S.
Becker, Vivien
Nalbach, Lisa
Gu, Yuan
Götz, Claudia
Menger, Michael D.
Laschke, Matthias W.
Ampofo, Emmanuel
Language: English
Title: Cells
Volume: 9
Issue: 6
Publisher/Platform: MDPI
Year of Publication: 2020
Free key words: CK2
NG2
promoter
pericytes
endothelial cells
angiogenesis
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Protein kinase CK2 is a crucial regulator of endothelial cell proliferation, migration and sprouting during angiogenesis. However, it is still unknown whether this kinase additionally affects the angiogenic activity of other vessel-associated cells. In this study, we investigated the effect of CK2 inhibition on primary human pericytes. We found that CK2 inhibition reduces the expression of nerve/glial antigen (NG)2, a crucial factor which is involved in angiogenic processes. Reporter gene assays revealed a 114 bp transcriptional active region of the human NG2 promoter, whose activity was decreased after CK2 inhibition. Functional analyses demonstrated that the pharmacological inhibition of CK2 by CX-4945 suppresses pericyte proliferation, migration, spheroid sprouting and the stabilization of endothelial tubes. Moreover, aortic rings of NG2−/− mice showed a significantly reduced vascular sprouting when compared to rings of NG2+/+ mice, indicating that NG2 is an important regulator of the angiogenic activity of pericytes. In vivo, implanted Matrigel plugs containing CX-4945-treated pericytes exhibited a lower microvessel density when compared to controls. These findings demonstrate that CK2 regulates the angiogenic activity of pericytes through NG2 gene expression. Hence, the inhibition of CK2 represents a promising anti-angiogenic strategy, because it does not only target endothelial cells, but also vessel-associated pericytes.
DOI of the first publication: 10.3390/cells9061546
Link to this record: urn:nbn:de:bsz:291--ds-313523
hdl:20.500.11880/30099
http://dx.doi.org/10.22028/D291-31352
ISSN: 2073-4409
Date of registration: 26-Nov-2020
Description of the related object: Supplementary Materials
Related object: https://www.mdpi.com/2073-4409/9/6/1546/s1
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
M - Medizinische Biochemie und Molekularbiologie
Professorship: M - Prof. Dr. Robert Ernst
M - Prof. Dr. Michael D. Menger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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