Please use this identifier to cite or link to this item:
doi:10.22028/D291-31352
Title: | Protein Kinase CK2 Regulates Nerve/Glial Antigen (NG)2-Mediated Angiogenic Activity of Human Pericytes |
Author(s): | Schmitt, Beate M. Boewe, Anne S. Becker, Vivien Nalbach, Lisa Gu, Yuan Götz, Claudia Menger, Michael D. Laschke, Matthias W. Ampofo, Emmanuel |
Language: | English |
Title: | Cells |
Volume: | 9 |
Issue: | 6 |
Publisher/Platform: | MDPI |
Year of Publication: | 2020 |
Free key words: | CK2 NG2 promoter pericytes endothelial cells angiogenesis |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Protein kinase CK2 is a crucial regulator of endothelial cell proliferation, migration and sprouting during angiogenesis. However, it is still unknown whether this kinase additionally affects the angiogenic activity of other vessel-associated cells. In this study, we investigated the effect of CK2 inhibition on primary human pericytes. We found that CK2 inhibition reduces the expression of nerve/glial antigen (NG)2, a crucial factor which is involved in angiogenic processes. Reporter gene assays revealed a 114 bp transcriptional active region of the human NG2 promoter, whose activity was decreased after CK2 inhibition. Functional analyses demonstrated that the pharmacological inhibition of CK2 by CX-4945 suppresses pericyte proliferation, migration, spheroid sprouting and the stabilization of endothelial tubes. Moreover, aortic rings of NG2−/− mice showed a significantly reduced vascular sprouting when compared to rings of NG2+/+ mice, indicating that NG2 is an important regulator of the angiogenic activity of pericytes. In vivo, implanted Matrigel plugs containing CX-4945-treated pericytes exhibited a lower microvessel density when compared to controls. These findings demonstrate that CK2 regulates the angiogenic activity of pericytes through NG2 gene expression. Hence, the inhibition of CK2 represents a promising anti-angiogenic strategy, because it does not only target endothelial cells, but also vessel-associated pericytes. |
DOI of the first publication: | 10.3390/cells9061546 |
Link to this record: | urn:nbn:de:bsz:291--ds-313523 hdl:20.500.11880/30099 http://dx.doi.org/10.22028/D291-31352 |
ISSN: | 2073-4409 |
Date of registration: | 26-Nov-2020 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/2073-4409/9/6/1546/s1 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Chirurgie M - Medizinische Biochemie und Molekularbiologie |
Professorship: | M - Prof. Dr. Robert Ernst M - Prof. Dr. Michael D. Menger |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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cells-09-01546.pdf | 3 MB | Adobe PDF | View/Open |
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