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doi:10.22028/D291-31352
Titel: | Protein Kinase CK2 Regulates Nerve/Glial Antigen (NG)2-Mediated Angiogenic Activity of Human Pericytes |
VerfasserIn: | Schmitt, Beate M. Boewe, Anne S. Becker, Vivien Nalbach, Lisa Gu, Yuan Götz, Claudia Menger, Michael D. Laschke, Matthias W. Ampofo, Emmanuel |
Sprache: | Englisch |
Titel: | Cells |
Bandnummer: | 9 |
Heft: | 6 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | CK2 NG2 promoter pericytes endothelial cells angiogenesis |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Protein kinase CK2 is a crucial regulator of endothelial cell proliferation, migration and sprouting during angiogenesis. However, it is still unknown whether this kinase additionally affects the angiogenic activity of other vessel-associated cells. In this study, we investigated the effect of CK2 inhibition on primary human pericytes. We found that CK2 inhibition reduces the expression of nerve/glial antigen (NG)2, a crucial factor which is involved in angiogenic processes. Reporter gene assays revealed a 114 bp transcriptional active region of the human NG2 promoter, whose activity was decreased after CK2 inhibition. Functional analyses demonstrated that the pharmacological inhibition of CK2 by CX-4945 suppresses pericyte proliferation, migration, spheroid sprouting and the stabilization of endothelial tubes. Moreover, aortic rings of NG2−/− mice showed a significantly reduced vascular sprouting when compared to rings of NG2+/+ mice, indicating that NG2 is an important regulator of the angiogenic activity of pericytes. In vivo, implanted Matrigel plugs containing CX-4945-treated pericytes exhibited a lower microvessel density when compared to controls. These findings demonstrate that CK2 regulates the angiogenic activity of pericytes through NG2 gene expression. Hence, the inhibition of CK2 represents a promising anti-angiogenic strategy, because it does not only target endothelial cells, but also vessel-associated pericytes. |
DOI der Erstveröffentlichung: | 10.3390/cells9061546 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-313523 hdl:20.500.11880/30099 http://dx.doi.org/10.22028/D291-31352 |
ISSN: | 2073-4409 |
Datum des Eintrags: | 26-Nov-2020 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Materials |
In Beziehung stehendes Objekt: | https://www.mdpi.com/2073-4409/9/6/1546/s1 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie M - Medizinische Biochemie und Molekularbiologie |
Professur: | M - Prof. Dr. Robert Ernst M - Prof. Dr. Michael D. Menger |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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cells-09-01546.pdf | 3 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons