Please use this identifier to cite or link to this item:
doi:10.22028/D291-32566
Title: | Loseolamycins: A Group of New Bioactive Alkylresorcinols Produced after Heterologous Expression of a Type III PKS from Micromonospora endolithica |
Author(s): | Lasch, Constanze Gummerlich, Nils Myronovskyi, Maksym Palusczak, Anja Zapp, Josef Luzhetskyy, Andriy |
Language: | English |
Title: | Molecules |
Volume: | 25 |
Issue: | 20 |
Publisher/Platform: | MDPI |
Year of Publication: | 2020 |
Free key words: | alkylresorcinol heterologous expression Micromonospora endolithica Streptomyces albus Del14 type III polyketide synthase biosynthetic gene |
DDC notations: | 500 Science 570 Life sciences, biology 600 Technology |
Publikation type: | Journal Article |
Abstract: | Natural products are a valuable source of biologically active compounds with potential applications in medicine and agriculture. Unprecedented scaffold diversity of natural products and biocatalysts from their biosynthetic pathways are of fundamental importance. Heterologous expression and refactoring of natural product biosynthetic pathways are generally regarded as a promising approach to discover new secondary metabolites of microbial origin. Here, we present the identification of a new group of alkylresorcinols after transcriptional activation and heterologous expression of the type III polyketide synthase of Micromonospora endolithica. The most abundant compounds loseolamycins A1 and A2 have been purified and their structures were elucidated by NMR. Loseolamycins contain an unusual branched hydroxylated aliphatic chain which is provided by the host metabolism and is incorporated as a starter fatty acid unit. The isolated loseolamycins show activity against gram-positive bacteria and inhibit the growth of the monocot weed Agrostis stolonifera in a germination assay. The biosynthetic pathway leading to the production of loseolamycins is proposed in this paper. |
DOI of the first publication: | 10.3390/molecules25204594 |
Link to this record: | urn:nbn:de:bsz:291--ds-325660 hdl:20.500.11880/30043 http://dx.doi.org/10.22028/D291-32566 |
ISSN: | 1420-3049 |
Date of registration: | 17-Nov-2020 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/1420-3049/25/20/4594/s1 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Professorship: | NT - Prof. Dr. Andriy Luzhetskyy |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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molecules-25-04594-v2.pdf | 945,91 kB | Adobe PDF | View/Open |
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