Please use this identifier to cite or link to this item: doi:10.22028/D291-32566
Title: Loseolamycins: A Group of New Bioactive Alkylresorcinols Produced after Heterologous Expression of a Type III PKS from Micromonospora endolithica
Author(s): Lasch, Constanze
Gummerlich, Nils
Myronovskyi, Maksym
Palusczak, Anja
Zapp, Josef
Luzhetskyy, Andriy
Language: English
Title: Molecules
Volume: 25
Issue: 20
Publisher/Platform: MDPI
Year of Publication: 2020
Free key words: alkylresorcinol
heterologous expression
Micromonospora endolithica
Streptomyces albus Del14
type III polyketide synthase
biosynthetic gene
DDC notations: 500 Science
570 Life sciences, biology
600 Technology
Publikation type: Journal Article
Abstract: Natural products are a valuable source of biologically active compounds with potential applications in medicine and agriculture. Unprecedented scaffold diversity of natural products and biocatalysts from their biosynthetic pathways are of fundamental importance. Heterologous expression and refactoring of natural product biosynthetic pathways are generally regarded as a promising approach to discover new secondary metabolites of microbial origin. Here, we present the identification of a new group of alkylresorcinols after transcriptional activation and heterologous expression of the type III polyketide synthase of Micromonospora endolithica. The most abundant compounds loseolamycins A1 and A2 have been purified and their structures were elucidated by NMR. Loseolamycins contain an unusual branched hydroxylated aliphatic chain which is provided by the host metabolism and is incorporated as a starter fatty acid unit. The isolated loseolamycins show activity against gram-positive bacteria and inhibit the growth of the monocot weed Agrostis stolonifera in a germination assay. The biosynthetic pathway leading to the production of loseolamycins is proposed in this paper.
DOI of the first publication: 10.3390/molecules25204594
Link to this record: urn:nbn:de:bsz:291--ds-325660
hdl:20.500.11880/30043
http://dx.doi.org/10.22028/D291-32566
ISSN: 1420-3049
Date of registration: 17-Nov-2020
Description of the related object: Supplementary Materials
Related object: https://www.mdpi.com/1420-3049/25/20/4594/s1
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Andriy Luzhetskyy
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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