Please use this identifier to cite or link to this item:
doi:10.22028/D291-29798
Title: | Effects of rAAV-Mediated sox9 Overexpression on the Biological Activities of Human Osteoarthritic Articular Chondrocytes in Their Intrinsic Three-Dimensional Environment |
Author(s): | Daniels, Oliver Frisch, Janina Venkatesan, Jagadeesh K. Rey-Rico, Ana Schmitt, Gertrud Cucchiarini, Magali |
Language: | English |
Title: | Journal of Clinical Medicine |
Volume: | 8 |
Issue: | 10 |
Publisher/Platform: | MDPI |
Year of Publication: | 2019 |
Free key words: | cartilage repair osteoarthritis rAAV SOX9 chondrocyte phenotype |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Gene therapy for osteoarthritis offers powerful, long-lasting tools that are well adapted to treat such a slow, progressive disorder, especially those therapies based on the clinically adapted recombinant adeno-associated viral (rAAV) vectors. Here, we examined the ability of an rAAV construct carrying a therapeutic sequence for the cartilage-specific SOX9 transcription factor to modulate the phenotype of human osteoarthritic articular chondrocytes compared with normal chondrocytes in a three-dimensional environment where the cells are embedded in their extracellular matrix. Successful <i>sox9</i> overexpression via rAAV was noted for at least 21 days, leading to the significant production of major matrix components (proteoglycans, type-II collagen) without affecting the proliferation of the cells, while the cells contained premature hypertrophic processes relative to control conditions (reporter rAAV-<i>lacZ</i> application, absence of vector treatment). These findings show the value of using rAAV to adjust the osteoarthritic phenotype when the chondrocytes are confined in their inherently altered environment and the possibility of impacting key cellular processes via gene therapy to remodel human osteoarthritic cartilage lesions. |
DOI of the first publication: | 10.3390/jcm8101637 |
Link to this record: | urn:nbn:de:bsz:291--ds-297980 hdl:20.500.11880/29136 http://dx.doi.org/10.22028/D291-29798 |
ISSN: | 2077-0383 |
Date of registration: | 13-May-2020 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Orthopädie |
Professorship: | M - Prof. Dr. Henning Madry |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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jcm-08-01637.pdf | 5,48 MB | Adobe PDF | View/Open |
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