Please use this identifier to cite or link to this item: doi:10.22028/D291-29798
Title: Effects of rAAV-Mediated sox9 Overexpression on the Biological Activities of Human Osteoarthritic Articular Chondrocytes in Their Intrinsic Three-Dimensional Environment
Author(s): Daniels, Oliver
Frisch, Janina
Venkatesan, Jagadeesh K.
Rey-Rico, Ana
Schmitt, Gertrud
Cucchiarini, Magali
Language: English
Title: Journal of Clinical Medicine
Volume: 8
Issue: 10
Publisher/Platform: MDPI
Year of Publication: 2019
Free key words: cartilage repair
osteoarthritis
rAAV
SOX9
chondrocyte phenotype
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Gene therapy for osteoarthritis offers powerful, long-lasting tools that are well adapted to treat such a slow, progressive disorder, especially those therapies based on the clinically adapted recombinant adeno-associated viral (rAAV) vectors. Here, we examined the ability of an rAAV construct carrying a therapeutic sequence for the cartilage-specific SOX9 transcription factor to modulate the phenotype of human osteoarthritic articular chondrocytes compared with normal chondrocytes in a three-dimensional environment where the cells are embedded in their extracellular matrix. Successful <i>sox9</i> overexpression via rAAV was noted for at least 21 days, leading to the significant production of major matrix components (proteoglycans, type-II collagen) without affecting the proliferation of the cells, while the cells contained premature hypertrophic processes relative to control conditions (reporter rAAV-<i>lacZ</i> application, absence of vector treatment). These findings show the value of using rAAV to adjust the osteoarthritic phenotype when the chondrocytes are confined in their inherently altered environment and the possibility of impacting key cellular processes via gene therapy to remodel human osteoarthritic cartilage lesions.
DOI of the first publication: 10.3390/jcm8101637
Link to this record: urn:nbn:de:bsz:291--ds-297980
hdl:20.500.11880/29136
http://dx.doi.org/10.22028/D291-29798
ISSN: 2077-0383
Date of registration: 13-May-2020
Faculty: M - Medizinische Fakultät
Department: M - Orthopädie
Professorship: M - Prof. Dr. Henning Madry
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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