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doi:10.22028/D291-29495
Title: | A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineered Corynebacterium glutamicum |
Author(s): | Rohles, Christina Maria Gläser, Lars Kohlstedt, Michael Gießelmann, Gideon Pearson, Samuel del Campo Bécares, Aránzazu Becker, Judith Wittmann, Christoph |
Language: | English |
Title: | Green chemistry : GC |
Volume: | 20 |
Issue: | 20 |
Startpage: | 4662 |
Endpage: | 4674 |
Publisher/Platform: | RSC |
Year of Publication: | 2018 |
Publikation type: | Journal Article |
Abstract: | In the present work, we established the bio-based production of glutarate, a carbon-5 dicarboxylic acid with recognized value for commercial plastics and other applications, using metabolically engineered Corynebacterium glutamicum. The mutant C. glutamicum AVA-2 served as a starting point for strain development, because it secreted small amounts of glutarate as a consequence of its engineered 5-aminovalerate pathway. Starting from AVA-2, we overexpressed 5-aminovalerate transaminase (gabT) and glutarate semialdehyde dehydrogenase (gabD) under the control of the constitutive tuf promoter to convert 5-aminovalerate further to glutarate. The created strain GTA-1 formed glutarate as a major product, but still secreted 5-aminovalerate as well. This bottleneck was tackled at the level of 5-aminovalerate re-import. The advanced strain GTA-4 overexpressed the newly discovered 5-aminovalerate importer NCgl0464 and formed glutarate from glucose in a yield of 0.27 mol mol−1. In a fed-batch process, GTA-4 produced more than 90 g L−1 glutarate from glucose and molasses based sugars in a yield of up to 0.70 mol mol−1 and a maximum productivity of 1.8 g L−1 h−1, while 5-aminovalerate was no longer secreted. The bio-based glutaric acid was purified to >99.9% purity. Interfacial polymerization and melt polymerization with hexamethylenediamine yielded bionylon-6,5, a polyamide with a unique structure. |
DOI of the first publication: | 10.1039/C8GC01901K |
URL of the first publication: | https://pubs.rsc.org/en/content/articlelanding/2018/gc/c8gc01901k#!divAbstract |
Link to this record: | hdl:20.500.11880/28209 http://dx.doi.org/10.22028/D291-29495 |
ISSN: | 1463-9270 1463-9262 |
Date of registration: | 25-Oct-2019 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Chemie NT - Biowissenschaften |
Professorship: | NT - Prof. Dr. Aránzazu del Campo NT - Prof. Dr. Christoph Wittmann |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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