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Titel: Molecular stiffness cues of an interpenetrating network hydrogel for cell adhesion
VerfasserIn: Li, Bin
Çolak, Arzu
Blass, Johanna
Han, Mitchell
Zhang, Jingnan
Zheng, Yijun
Jiang, Qiyang
Bennewitz, Roland
del Campo Bécares, Aránzazu
Sprache: Englisch
Titel: Materials today bio
Bandnummer: 15
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2022
Freie Schlagwörter: IPNs
Nanomechanics
Mechanotransduction
Cell adhesion
AFM
DDC-Sachgruppe: 600 Technik
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Understanding cells' response to the macroscopic and nanoscale properties of biomaterials requires studies in model systems with the possibility to tailor their mechanical properties and different length scales. Here, we describe an interpenetrating network (IPN) design based on a stiff PEGDA host network interlaced within a soft 4-arm PEG-Maleimide/thiol (guest) network. We quantify the nano- and bulk mechanical behavior of the IPN and the single network hydrogels by single-molecule force spectroscopy and rheological measurements. The IPN presents different mechanical cues at the molecular scale, depending on which network is linked to the probe, but the same mechanical properties at the macroscopic length scale as the individual host network. Cells attached to the interpenetrating (guest) network of the IPN or to the single network (SN) PEGDA hydrogel modified with RGD adhesive ligands showed comparable attachment and spreading areas, but cells attached to the guest network of the IPN, with lower molecular stiffness, showed a larger number and size of focal adhesion complexes and a higher concentration of the Hippo pathway effector Yes-associated protein (YAP) than cells linked to the PEGDA single network. The observations indicate that cell adhesion to the IPN hydrogel through the network with lower molecular stiffness proceeds effectively as if a higher ligand density is offered. We claim that IPNs can be used to decipher how changes in ECM design and connectivity at the local scale affect the fate of cells cultured on biomaterials.
DOI der Erstveröffentlichung: 10.1016/j.mtbio.2022.100323
URL der Erstveröffentlichung: https://www.sciencedirect.com/science/article/pii/S2590006422001211
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-368791
hdl:20.500.11880/34249
http://dx.doi.org/10.22028/D291-36879
ISSN: 2590-0064
Datum des Eintrags: 8-Nov-2022
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons