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doi:10.22028/D291-36361
Titel: | Intravenous propofol, ketamine (ketofol) and rocuronium after sevoflurane induction provides long lasting anesthesia in ventilated rats |
VerfasserIn: | Kiefer, Daniel Müller-Wirtz, Lukas M. Maurer, Felix Hüppe, Tobias Mathes, Alexander M. Volk, Thomas Kreuer, Sascha Fink, Tobias |
Sprache: | Englisch |
Titel: | Experimental Animals |
Bandnummer: | 71 |
Heft: | 2 |
Seiten: | 231-239 |
Verlag/Plattform: | Japanese Association for Laboratory Animal Science |
Erscheinungsjahr: | 2021 |
Freie Schlagwörter: | anesthetics plasma concentration rat anesthesia techniques rat inhalation anesthesia rat long time anesthesia rat ventilation |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Rats are commonly used animals for laboratory experiments and many experiments require general anesthesia. However, the lack of published and reproducible intravenous anesthesia protocols for rats results in unnecessary animal use to establish new anesthesia techniques across institutions. We therefore developed an anesthesia protocol with propofol, ketamine, and rocuronium for mechanically ventilated rats, and evaluated vital parameters and plasma concentrations. 15 male Sprague-Dawley rats underwent inhalation induction with sevoflurane and tracheal, venous and arterial cannulation. After established venous access, sevoflurane was substituted by propofol and ketamine (ketofol). Rocuronium was added under mechanical ventilation for 7 h. Drug dosages were stepwise reduced to prevent accumulation. All animals survived the observation period and showed adequate depth of anesthesia. Mean arterial pressure and heart rate remained within normal ranges. Median propofol plasma concentrations remained stable: 1, 4, 7 h: 2.0 (interquartile range (IQR): 1.8–2.2), 2.1 (1.8–2.2), 1.8 (1.6–2.1) µg/ml, whereas median ketamine concentrations slightly differed after 7 h compared to 1 h: 1, 4, 7 h: 3.7 (IQR: 3.5–4.5), 3.8 (3.3–4.1), 3.8 (3.0–4.1) µg/ml. Median rocuronium plasma concentrations were lower after 4 and 7 h compared to 1 h: 1, 4, 7 h: 3.9 (IQR: 3.5–4.9), 3.2 (2.7–3.3), 3.0 (2.4–3.4) µg/ml. Our anesthesia protocol provides stable and reliable anesthesia in mechanically ventilated rats for several hours. |
DOI der Erstveröffentlichung: | 10.1538/expanim.21-0147 |
URL der Erstveröffentlichung: | https://doi.org/10.1538/expanim.21-0147 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-363615 hdl:20.500.11880/33017 http://dx.doi.org/10.22028/D291-36361 |
ISSN: | 1881-7122 1341-1357 |
Datum des Eintrags: | 3-Jun-2022 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary material |
In Beziehung stehendes Objekt: | https://www.jstage.jst.go.jp/article/expanim/71/2/71_21-0147/_supplement/_download/71_21-0147_1.pdf |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Anästhesiologie |
Professur: | M - Prof. Dr. Thomas Volk |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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expanim-71-231.pdf | 842,27 kB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons