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Titel: Intravenous propofol, ketamine (ketofol) and rocuronium after sevoflurane induction provides long lasting anesthesia in ventilated rats
VerfasserIn: Kiefer, Daniel
Müller-Wirtz, Lukas M.
Maurer, Felix
Hüppe, Tobias
Mathes, Alexander M.
Volk, Thomas
Kreuer, Sascha
Fink, Tobias
Sprache: Englisch
Titel: Experimental Animals
Bandnummer: 71
Heft: 2
Seiten: 231-239
Verlag/Plattform: Japanese Association for Laboratory Animal Science
Erscheinungsjahr: 2021
Freie Schlagwörter: anesthetics plasma concentration
rat anesthesia techniques
rat inhalation anesthesia
rat long time anesthesia
rat ventilation
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Rats are commonly used animals for laboratory experiments and many experiments require general anesthesia. However, the lack of published and reproducible intravenous anesthesia protocols for rats results in unnecessary animal use to establish new anesthesia techniques across institutions. We therefore developed an anesthesia protocol with propofol, ketamine, and rocuronium for mechanically ventilated rats, and evaluated vital parameters and plasma concentrations. 15 male Sprague-Dawley rats underwent inhalation induction with sevoflurane and tracheal, venous and arterial cannulation. After established venous access, sevoflurane was substituted by propofol and ketamine (ketofol). Rocuronium was added under mechanical ventilation for 7 h. Drug dosages were stepwise reduced to prevent accumulation. All animals survived the observation period and showed adequate depth of anesthesia. Mean arterial pressure and heart rate remained within normal ranges. Median propofol plasma concentrations remained stable: 1, 4, 7 h: 2.0 (interquartile range (IQR): 1.8–2.2), 2.1 (1.8–2.2), 1.8 (1.6–2.1) µg/ml, whereas median ketamine concentrations slightly differed after 7 h compared to 1 h: 1, 4, 7 h: 3.7 (IQR: 3.5–4.5), 3.8 (3.3–4.1), 3.8 (3.0–4.1) µg/ml. Median rocuronium plasma concentrations were lower after 4 and 7 h compared to 1 h: 1, 4, 7 h: 3.9 (IQR: 3.5–4.9), 3.2 (2.7–3.3), 3.0 (2.4–3.4) µg/ml. Our anesthesia protocol provides stable and reliable anesthesia in mechanically ventilated rats for several hours.
DOI der Erstveröffentlichung: 10.1538/expanim.21-0147
URL der Erstveröffentlichung: https://doi.org/10.1538/expanim.21-0147
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-363615
hdl:20.500.11880/33017
http://dx.doi.org/10.22028/D291-36361
ISSN: 1881-7122
1341-1357
Datum des Eintrags: 3-Jun-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplementary material
In Beziehung stehendes Objekt: https://www.jstage.jst.go.jp/article/expanim/71/2/71_21-0147/_supplement/_download/71_21-0147_1.pdf
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Anästhesiologie
Professur: M - Prof. Dr. Thomas Volk
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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