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Titel: Quantification of Volatile Acetone Oligomers Using Ion-Mobility Spectrometry
VerfasserIn: Hüppe, Tobias
Lorenz, Dominik
Maurer, Felix
Fink, Tobias
Klumpp, Ramona
Kreuer, Sascha
Sprache: Englisch
Titel: Journal of Analytical Methods in Chemistry
Bandnummer: 2021
Verlag/Plattform: Hindawi
Erscheinungsjahr: 2021
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Background. Volatile acetone is a potential biomarker that is elevated in various disease states. Measuring acetone in exhaled breath is complicated by the fact that the molecule might be present as both monomers and dimers, but in inconsistent ratios. Ignoring the molecular form leads to incorrect measured concentrations. Our first goal was to evaluate the monomer-dimer ratio in ambient air, critically ill patients, and rats. Our second goal was to confirm the accuracy of the combined (monomer and dimer) analysis by comparison to a reference calibration system. Methods. Volatile acetone intensities from exhaled air of ten intubated, critically ill patients, and ten ventilated Sprague-Dawley rats were recorded using ion-mobility spectrometry. Acetone concentrations in ambient air in an intensive care unit and in a laboratory were determined over 24 hours. )e calibration reference was pure acetone vaporized by a gas generator at concentrations from 5 to 45 ppbv (parts per billion by volume). Results. Acetone concentrations in ambient laboratory air were only slightly greater (5.6 ppbv; 95% CI 5.1–6.2) than in ambient air in an intensive care unit (5.1 ppbv; 95% CI 4.4–5.5; p < 0.001). Exhaled acetone concentrations were only slightly greater in rats (10.3 ppbv; 95% CI 9.7–10.9) than in critically ill patients (9.5 ppbv; 95% CI 7.9–11.1; p < 0.001). Vaporization yielded acetone monomers (1.3–5.3 mV) and dimers (1.4–621 mV). Acetone concentrations (ppbv) and corresponding acetone monomer and dimer intensities (mV) revealed a high coefficient of determination (R2 � 0.96). )e calibration curve for acetone concentration (ppbv) and total acetone (monomers added to twice the dimers; mV) was described by the exponential growth 3-parameter model, with an R2 � 0.98. Conclusion. )e ratio of acetone monomer and dimer is inconsistent and varies in ambient air from place-to-place and across individual humans and rats. Monomers and dimers must therefore be considered when quantifying acetone. Combining the two accurately assesses total volatile acetone.
DOI der Erstveröffentlichung: 10.1155/2021/6638036
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-347185
hdl:20.500.11880/31778
http://dx.doi.org/10.22028/D291-34718
ISSN: 2090-8873
2090-8865
Datum des Eintrags: 21-Sep-2021
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Anästhesiologie
Professur: M - Prof. Dr. Thomas Volk
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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