Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
doi:10.22028/D291-34299
Titel: | The Erythrocyte Sedimentation Rate and Its Relation to Cell Shape and Rigidity of Red Blood Cells from Chorea-Acanthocytosis Patients in an Off-Label Treatment with Dasatinib |
VerfasserIn: | Rabe, Antonia Kihm, Alexander Darras, Alexis Peikert, Kevin Simionato, Greta Dasanna, Anil Kumar Glaß, Hannes Geisel, Jürgen Quint, Stephan Danek, Adrian Wagner, Christian Fedosov, Dmitry A. Hermann, Andreas Kästner, Lars |
Sprache: | Englisch |
Titel: | Biomolecules |
Bandnummer: | 11 |
Heft: | 5 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2021 |
Freie Schlagwörter: | acanthocytes dasatinib erythrocyte sedimentation rate 3D shapes microfluidics phase diagram cell rigidity mesoscopic modeling |
DDC-Sachgruppe: | 530 Physik 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Background: Chorea-acanthocytosis (ChAc) is a rare hereditary neurodegenerative disease with deformed red blood cells (RBCs), so-called acanthocytes, as a typical marker of the disease. Erythrocyte sedimentation rate (ESR) was recently proposed as a diagnostic biomarker. To date, there is no treatment option for affected patients, but promising therapy candidates, such as dasatinib, a Lyn-kinase inhibitor, have been identified. Methods: RBCs of two ChAc patients during and after dasatinib treatment were characterized by the ESR, clinical hematology parameters and the 3D shape classification in stasis based on an artificial neural network. Furthermore, mathematical modeling was performed to understand the contribution of cell morphology and cell rigidity to the ESR. Microfluidic measurements were used to compare the RBC rigidity between ChAc patients and healthy controls. Results: The mechano-morphological characterization of RBCs from two ChAc patients in an off-label treatment with dasatinib revealed differences in the ESR and the acanthocyte count during and after the treatment period, which could not directly be related to each other. Clinical hematology parameters were in the normal range. Mathematical modeling indicated that RBC rigidity is more important for delayed ESR than cell shape. Microfluidic experiments confirmed a higher rigidity in the normocytes of ChAc patients compared to healthy controls. Conclusions: The results increase our understanding of the role of acanthocytes and their associated properties in the ESR, but the data are too sparse to answer the question of whether the ESR is a suitable biomarker for treatment success, whereas a correlation between hematological and neuronal phenotype is still subject to verification. |
DOI der Erstveröffentlichung: | 10.3390/biom11050727 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-342999 hdl:20.500.11880/31486 http://dx.doi.org/10.22028/D291-34299 |
ISSN: | 2218-273X |
Datum des Eintrags: | 6-Jul-2021 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Materials |
In Beziehung stehendes Objekt: | https://www.mdpi.com/article/10.3390/biom11050727/s1 |
Fakultät: | M - Medizinische Fakultät NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | M - Innere Medizin NT - Physik |
Professur: | M - Keiner Professur zugeordnet NT - Prof. Dr. Christian Wagner |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
biomolecules-11-00727-v2.pdf | 5,63 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons