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doi:10.22028/D291-34261
Titel: | Quantitative Proteomics and Differential Protein Abundance Analysis after Depletion of Putative mRNA Receptors in the ER Membrane of Human Cells Identifies Novel Aspects of mRNA Targeting to the ER |
VerfasserIn: | Bhadra, Pratiti Schorr, Stefan Lerner, Monika Nguyen, Duy Dudek, Johanna Förster, Friedrich Helms, Volkhard Lang, Sven Zimmermann, Richard |
Sprache: | Englisch |
Titel: | Molecules |
Bandnummer: | 26 |
Heft: | 12 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2021 |
Freie Schlagwörter: | endoplasmic reticulum mRNA targeting protein targeting protein import membrane protein insertion protein translocation Sec61 complex TIGER domain label-free quantitative mass spectrometry differential protein abundance analysis |
DDC-Sachgruppe: | 500 Naturwissenschaften 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | In human cells, one-third of all polypeptides enter the secretory pathway at the endoplasmic reticulum (ER). The specificity and efficiency of this process are guaranteed by targeting of mRNAs and/or polypeptides to the ER membrane. Cytosolic SRP and its receptor in the ER membrane facilitate the cotranslational targeting of most ribosome-nascent precursor polypeptide chain (RNC) complexes together with the respective mRNAs to the Sec61 complex in the ER membrane. Alternatively, fully synthesized precursor polypeptides are targeted to the ER membrane post-translationally by either the TRC, SND, or PEX19/3 pathway. Furthermore, there is targeting of mRNAs to the ER membrane, which does not involve SRP but involves mRNA- or RNC-binding proteins on the ER surface, such as RRBP1 or KTN1. Traditionally, the targeting reactions were studied in cell-free or cellular assays, which focus on a single precursor polypeptide and allow the conclusion of whether a certain precursor can use a certain pathway. Recently, cellular approaches such as proximity-based ribosome profiling or quantitative proteomics were employed to address the question of which precursors use certain pathways under physiological conditions. Here, we combined siRNA-mediated depletion of putative mRNA receptors in HeLa cells with label-free quantitative proteomics and differential protein abundance analysis to characterize RRBP1- or KTN1- involving precursors and to identify possible genetic interactions between the various targeting pathways. Furthermore, we discuss the possible implications on the so-called TIGER domains and critically discuss the pros and cons of this experimental approach. |
DOI der Erstveröffentlichung: | 10.3390/molecules26123591 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-342615 hdl:20.500.11880/31459 http://dx.doi.org/10.22028/D291-34261 |
ISSN: | 1420-3049 |
Datum des Eintrags: | 1-Jul-2021 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Materials |
In Beziehung stehendes Objekt: | https://www.mdpi.com/1420-3049/26/12/3591/s1 |
Fakultät: | M - Medizinische Fakultät ZE - Zentrale Einrichtungen |
Fachrichtung: | M - Medizinische Biochemie und Molekularbiologie ZE - Zentrum für Bioinformatik(ZBI) |
Professur: | M - Keiner Professur zugeordnet ZE - Sonstige |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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molecules-26-03591-v2.pdf | 40,44 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons