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doi:10.22028/D291-32859
Titel: | EGFR Expression in HER2-Driven Breast Cancer Cells |
VerfasserIn: | Weinberg, Florian Peckys, Diana B. de Jonge, Niels |
Sprache: | Englisch |
Titel: | International Journal of Molecular Sciences |
Bandnummer: | 21 |
Heft: | 23 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | EGFR HER2 electron microscopy single molecule correlative microscopy dual labeling |
DDC-Sachgruppe: | 500 Naturwissenschaften 570 Biowissenschaften, Biologie 600 Technik 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The epidermal growth factor receptor HER2 is overexpressed in 20% of breast cancer cases. HER2 is an orphan receptor that is activated ligand-independently by homodimerization. In addition, HER2 is able to heterodimerize with EGFR, HER3, and HER4. Heterodimerization has been proposed as a mechanism of resistance to therapy for HER2 overexpressing breast cancer. Here, a method is presented for the simultaneous detection of individual EGFR and HER2 receptors in the plasma membrane of breast cancer cells via specific labeling with quantum dot nanoparticles (QDs). Correlative fluorescence microscopy and liquid phase electron microscopy were used to analyze the plasma membrane expression levels of both receptors in individual intact cells. Fluorescent single-cell analysis of SKBR3 breast cancer cells dual-labeled for EGFR and HER2 revealed a heterogeneous expression for receptors within both the cell population as well as within individual cells. Subsequent electron microscopy of individual cells allowed the determination of individual receptors label distributions. QD-labeled EGFR was observed with a surface density of (0.5–5) × 101 QDs/µm2 , whereas labeled HER2 expression was higher ranging from (2–10) × 102 QDs/µm2 . Although most SKBR3 cells expressed low levels of EGFR, an enrichment was observed at large plasma membrane protrusions, and amongst a newly discovered cellular subpopulation termed EGFR-enriched cells. |
DOI der Erstveröffentlichung: | 10.3390/ijms21239008 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-328591 hdl:20.500.11880/30291 http://dx.doi.org/10.22028/D291-32859 |
ISSN: | 1422-0067 |
Datum des Eintrags: | 22-Dez-2020 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Materials |
In Beziehung stehendes Objekt: | http://www.mdpi.com/1422-0067/21/23/9008/s1 |
Fakultät: | M - Medizinische Fakultät NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | M - Biophysik NT - Physik |
Professur: | NT - Keiner Professur zugeordnet M - Keiner Professur zugeordnet |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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ijms-21-09008-v2.pdf | 15,55 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons