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Titel: Macrophages promote network formation and maturation of transplanted adipose tissue-derived microvascular fragments
VerfasserIn: Später, Thomas
Menger, Maximilian M.
Nickels, Ruth M
Menger, Michael D.
Laschke, Matthias W.
Sprache: Englisch
Titel: Journal of Tissue Engineering
Bandnummer: 11
Verlag/Plattform: SAGE
Erscheinungsjahr: 2020
Freie Schlagwörter: Tissue engineering
microvascular fragments
scaffold
vascularization
clodronate
macrophage depletion
immune cells
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Adipose tissue-derived microvascular fragments rapidly reassemble into microvascular networks within implanted scaffolds. Herein, we analyzed the contribution of macrophages to this process. C57BL/6 mice received clodronate (clo)-containing liposomes for macrophage depletion, whereas animals treated with phosphate-buffered-saline-containing liposomes served as controls. Microvascular fragments were isolated from clo- and phosphate-buffered-saline-treated donor mice and seeded onto collagen-glycosaminoglycan matrices, which were implanted into dorsal skinfold chambers of clo- and phosphate-buffered-saline-treated recipient mice. The implants' vascularization and incorporation were analyzed by stereomicroscopy, intravital fluorescence microscopy, histology, and immunohistochemistry. Compared to controls, matrices within clo-treated animals exhibited a significantly reduced functional microvessel density. Moreover, they contained a lower fraction of microvessels with an α-smooth muscle actin (SMA)+ cell layer, indicating impaired vessel maturation. This was associated with a deteriorated implant incorporation. These findings demonstrate that macrophages not only promote the reassembly of microvascular fragments into microvascular networks, but also improve their maturation during this process.
DOI der Erstveröffentlichung: 10.1177/2041731420911816
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-328647
hdl:20.500.11880/30186
http://dx.doi.org/10.22028/D291-32864
ISSN: 2041-7314
Datum des Eintrags: 10-Dez-2020
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
M - Prof. Dr. Tim Pohlemann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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