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doi:10.22028/D291-32864
Titel: | Macrophages promote network formation and maturation of transplanted adipose tissue-derived microvascular fragments |
VerfasserIn: | Später, Thomas Menger, Maximilian M. Nickels, Ruth M Menger, Michael D. Laschke, Matthias W. |
Sprache: | Englisch |
Titel: | Journal of Tissue Engineering |
Bandnummer: | 11 |
Verlag/Plattform: | SAGE |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | Tissue engineering microvascular fragments scaffold vascularization clodronate macrophage depletion immune cells |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Adipose tissue-derived microvascular fragments rapidly reassemble into microvascular networks within implanted scaffolds. Herein, we analyzed the contribution of macrophages to this process. C57BL/6 mice received clodronate (clo)-containing liposomes for macrophage depletion, whereas animals treated with phosphate-buffered-saline-containing liposomes served as controls. Microvascular fragments were isolated from clo- and phosphate-buffered-saline-treated donor mice and seeded onto collagen-glycosaminoglycan matrices, which were implanted into dorsal skinfold chambers of clo- and phosphate-buffered-saline-treated recipient mice. The implants' vascularization and incorporation were analyzed by stereomicroscopy, intravital fluorescence microscopy, histology, and immunohistochemistry. Compared to controls, matrices within clo-treated animals exhibited a significantly reduced functional microvessel density. Moreover, they contained a lower fraction of microvessels with an α-smooth muscle actin (SMA)+ cell layer, indicating impaired vessel maturation. This was associated with a deteriorated implant incorporation. These findings demonstrate that macrophages not only promote the reassembly of microvascular fragments into microvascular networks, but also improve their maturation during this process. |
DOI der Erstveröffentlichung: | 10.1177/2041731420911816 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-328647 hdl:20.500.11880/30186 http://dx.doi.org/10.22028/D291-32864 |
ISSN: | 2041-7314 |
Datum des Eintrags: | 10-Dez-2020 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie |
Professur: | M - Prof. Dr. Michael D. Menger M - Prof. Dr. Tim Pohlemann |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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2041731420911816.pdf | Macrophages promote network formation and maturation of transplanted adipose tissue–derived microvascular fragments | 5,34 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons