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Titel: Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A
VerfasserIn: Voitsekhovskaia, Irina
Paulus, Constanze
Dahlem, Charlotte
Rebets, Yuriy
Nadmid, Suvd
Zapp, Josef
Axenov-Gribanov, Denis
Rückert, Christian
Timofeyev, Maxim
Kalinowski, Jörn
Kiemer, Alexandra K.
Luzhetskyy, Andriy
Sprache: Englisch
Titel: Microorganisms
Bandnummer: 8
Heft: 5
Verlag/Plattform: MDPI
Erscheinungsjahr: 2020
Freie Schlagwörter: natural products
angucycline
aquayamycin
glycosyltransferase
Lake Baikal
Streptomyces
DDC-Sachgruppe: 500 Naturwissenschaften
610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Natural products produced by bacteria found in unusual and poorly studied ecosystems, such as Lake Baikal, represent a promising source of new valuable drug leads. Here we report the isolation of a new Streptomyces sp. strain IB201691-2A from the Lake Baikal endemic mollusk Benedictia baicalensis. In the course of an activity guided screening three new angucyclines, named baikalomycins A–C, were isolated and characterized, highlighting the potential of poorly investigated ecological niches. Besides that, the strain was found to accumulate large quantities of rabelomycin and 5-hydroxy-rabelomycin, known shunt products in angucyclines biosynthesis. Baikalomycins A–C demonstrated varying degrees of anticancer activity. Rabelomycin and 5-hydroxy-rabelomycin further demonstrated antiproliferative activities. The structure elucidation showed that baikalomycin A is a modified aquayamycin with β-d-amicetose and two additional hydroxyl groups at unusual positions (6a and 12a) of aglycone. Baikalomycins B and C have alternating second sugars attached, α-l-amicetose and α-l-aculose, respectively. The gene cluster for baikalomycins biosynthesis was identified by genome mining, cloned using a transformation-associated recombination technique and successfully expressed in S. albus J1074. It contains a typical set of genes responsible for an angucycline core assembly, all necessary genes for the deoxy sugars biosynthesis, and three genes coding for the glycosyltransferase enzymes. Heterologous expression and deletion experiments allowed to assign the function of glycosyltransferases involved in the decoration of baikalomycins aglycone.
DOI der Erstveröffentlichung: 10.3390/microorganisms8050680
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-310543
hdl:20.500.11880/29922
http://dx.doi.org/10.22028/D291-31054
ISSN: 2076-2607
Datum des Eintrags: 30-Okt-2020
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Materials
In Beziehung stehendes Objekt: https://www.mdpi.com/2076-2607/8/5/680/s1
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Pharmazie
Professur: NT - Prof. Dr. Alexandra K. Kiemer
NT - Prof. Dr. Andriy Luzhetskyy
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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