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Titel: Insulin-like growth factor 1 stimulates the angiogenic activity of adipose tissue-derived microvascular fragments
VerfasserIn: Laschke, Matthias W.
Kontaxi, Elena
Scheuer, Claudia
Heß, Alexander
Karschnia, Philipp
Menger, Michael D.
Sprache: Englisch
Titel: Journal of Tissue Engineering
Bandnummer: 10
Verlag/Plattform: SAGE
Erscheinungsjahr: 2019
Freie Schlagwörter: Tissue engineering
microvascular fragments
insulin-like growth factor 1
insulin-like growth factor–binding protein 4
angiogenesis
vascularization
scaffold
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Angiogenesis in adipose tissue is promoted by insulin-like growth factor 1 signaling. We analyzed whether this regulatory mechanism also improves the angiogenic activity of adipose tissue-derived microvascular fragments. Murine adipose tissue-derived microvascular fragments were cultivated for 24 h in the University of Wisconsin (UW) solution supplemented with vehicle, insulin-like growth factor 1, or a combination of insulin-like growth factor 1 and insulin-like growth factor-binding protein 4. Subsequently, we assessed their cellular composition, viability, proliferation, and growth factor expression. Moreover, cultivated adipose tissue-derived microvascular fragments were seeded onto collagen-glycosaminoglycan scaffolds, which were implanted into dorsal skinfold chambers to study their vascularization and incorporation. Insulin-like growth factor 1 increased the viability and growth factor expression of adipose tissue-derived microvascular fragments without affecting their cellular composition and proliferation. Accordingly, scaffolds containing insulin-like growth factor 1-stimulated adipose tissue-derived microvascular fragments exhibited an enhanced in vivo vascularization and incorporation. These positive insulin-like growth factor 1 effects were reversed by additional exposure of adipose tissue-derived microvascular fragments to insulin-like growth factor-binding protein 4. Our findings indicate that insulin-like growth factor 1 stimulation of adipose tissue-derived microvascular fragments is suitable to improve their vascularization capacity.
DOI der Erstveröffentlichung: 10.1177/2041731419879837
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-312651
hdl:20.500.11880/29295
http://dx.doi.org/10.22028/D291-31265
ISSN: 2041-7314
Datum des Eintrags: 22-Jun-2020
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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