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doi:10.22028/D291-31265
Titel: | Insulin-like growth factor 1 stimulates the angiogenic activity of adipose tissue-derived microvascular fragments |
VerfasserIn: | Laschke, Matthias W. Kontaxi, Elena Scheuer, Claudia Heß, Alexander Karschnia, Philipp Menger, Michael D. |
Sprache: | Englisch |
Titel: | Journal of Tissue Engineering |
Bandnummer: | 10 |
Verlag/Plattform: | SAGE |
Erscheinungsjahr: | 2019 |
Freie Schlagwörter: | Tissue engineering microvascular fragments insulin-like growth factor 1 insulin-like growth factor–binding protein 4 angiogenesis vascularization scaffold |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Angiogenesis in adipose tissue is promoted by insulin-like growth factor 1 signaling. We analyzed whether this regulatory mechanism also improves the angiogenic activity of adipose tissue-derived microvascular fragments. Murine adipose tissue-derived microvascular fragments were cultivated for 24 h in the University of Wisconsin (UW) solution supplemented with vehicle, insulin-like growth factor 1, or a combination of insulin-like growth factor 1 and insulin-like growth factor-binding protein 4. Subsequently, we assessed their cellular composition, viability, proliferation, and growth factor expression. Moreover, cultivated adipose tissue-derived microvascular fragments were seeded onto collagen-glycosaminoglycan scaffolds, which were implanted into dorsal skinfold chambers to study their vascularization and incorporation. Insulin-like growth factor 1 increased the viability and growth factor expression of adipose tissue-derived microvascular fragments without affecting their cellular composition and proliferation. Accordingly, scaffolds containing insulin-like growth factor 1-stimulated adipose tissue-derived microvascular fragments exhibited an enhanced in vivo vascularization and incorporation. These positive insulin-like growth factor 1 effects were reversed by additional exposure of adipose tissue-derived microvascular fragments to insulin-like growth factor-binding protein 4. Our findings indicate that insulin-like growth factor 1 stimulation of adipose tissue-derived microvascular fragments is suitable to improve their vascularization capacity. |
DOI der Erstveröffentlichung: | 10.1177/2041731419879837 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-312651 hdl:20.500.11880/29295 http://dx.doi.org/10.22028/D291-31265 |
ISSN: | 2041-7314 |
Datum des Eintrags: | 22-Jun-2020 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie |
Professur: | M - Prof. Dr. Michael D. Menger |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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2041731419879837.pdf | Insulin-like growth factor 1 stimulates the angiogenic activity of adipose tissue–derived microvascular fragments | 4,84 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons