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Titel: The Evolution of Erythrocytes Becoming Red in Respect to Fluorescence
VerfasserIn: Hertz, Laura
Ruppenthal, Sandra
Simionato, Greta
Quint, Stephan
Kihm, Alexander
Abay, Asena
Petkova-Kirova, Polina
Boehm, Ulrich
Weissgerber, Petra
Wagner, Christian
Laschke, Matthias W.
Kaestner, Lars
Sprache: Englisch
Titel: Frontiers in Physiology
Bandnummer: 10
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2019
Freie Schlagwörter: mouse model
transfusion
fluorescent protein
intravital microscopy
imaging
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Very young red blood cells, namely reticulocytes, can be quite easily recognized and labeled by cluster of differentiation antibodies (CD71, transferrin receptor) or by staining remnant RNA with thiazol orange. In contrast, age specific erythrocyte labeling is more difficult in later periods of their life time. While erythrocytes contain band 4.1 protein, a molecular clock, so far it has not been possible to read this clock on individual cells. One concept to track erythrocytes during their life time is to mark them when they are young, either directly in vivo or ex vivo followed by a transfusion. Several methods like biotinylation, use of isotopes or fluorescent labeling have proved to be useful experimental approaches but also have several inherent disadvantages. Genetic engineering of mice provides additional options to express fluorescent proteins in erythrocytes. To allow co-staining with popular green fluorescent dyes like Fluo-4 or other fluorescein-based dyes, we bred a mouse line expressing a tandem red fluorescent protein (tdRFP). Within this Brief Research Report, we provide the initial characterisation of this mouse line and show application examples ranging from transfusion experiments and intravital microscopy to multicolour flow cytometry and confocal imaging. We provide a versatile new tool for erythrocyte research and discuss a range of experimental opportunities to study membrane processes and other aspects of erythrocyte development and aging with help of these animals.
DOI der Erstveröffentlichung: 10.3389/fphys.2019.00753
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-311811
hdl:20.500.11880/29247
http://dx.doi.org/10.22028/D291-31181
ISSN: 1664-042X
Datum des Eintrags: 15-Jun-2020
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Material
In Beziehung stehendes Objekt: https://www.frontiersin.org/articles/10.3389/fphys.2019.00753/full#supplementary-material
Fakultät: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: M - Anatomie und Zellbiologie
NT - Physik
Professur: M - Keiner Professur zugeordnet
NT - Prof. Dr. Christian Wagner
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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