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doi:10.22028/D291-31037
Titel: | Translation of two-photon microscopy to the clinic: multimodal multiphoton CARS tomography of in vivo human skin |
VerfasserIn: | König, Karsten Breunig, Hans Georg Goncalves Batista, Ana Maria Schindele, Andreas Zieger, Michael Kaatz, Martin |
Sprache: | Englisch |
Titel: | Journal of Biomedical Optics |
Bandnummer: | 25 |
Heft: | 1 |
Verlag/Plattform: | SPIE |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | Skin Second-harmonic generation In vivo imaging Luminescence Two photon excitation microscopy Femtosecond phenomena Fluorescence lifetime imaging Biopsy CARS tomography Microscopes |
DDC-Sachgruppe: | 600 Technik |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Two-photon microscopes have been successfully translated into clinical imaging tools to obtain high-resolution optical biopsies for in vivo histology. We report on clinical multiphoton coherent anti-Stokes Raman spectroscopy (CARS) tomography based on two tunable ultrashort near-infrared laser beams for label-free in vivo multimodal skin imaging. The multiphoton biopsies were obtained with the compact tomograph “MPTflex-CARS” using a photonic crystal fiber, an optomechanical articulated arm, and a four-detector-360 deg measurement head. The multiphoton tomograph has been employed to patients in a hospital with diseased skin. The clinical study involved 16 subjects, 8 patients with atopic dermatitis, 4 patients with psoriasis vulgaris, and 4 volunteers served as control. Two-photon cellular autofluorescence lifetime, second harmonic generation (SHG) of collagen, and CARS of intratissue lipids/proteins have been detected with single-photon sensitivity, submicron spatial resolution, and picosecond temporal resolution. The most important signal was the autofluorescence from nicotinamide adenine dinucleotide [NAD(P)H]. The SHG signal from collagen was mainly used to detect the epidermal–dermal junction and to calculate the ratio elastin/collagen. The CARS/Raman signal provided add-on information. Based on this view on the disease-affected skin on a subcellular level, skin areas affected by dermatitis and by psoriasis could be clearly identified. Multimodal multiphoton tomographs may become important label-free clinical high-resolution imaging tools for in vivo skin histology to realize rapid early diagnosis as well as treatment control. |
DOI der Erstveröffentlichung: | 10.1117/1.JBO.25.1.014515 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-310373 hdl:20.500.11880/29189 http://dx.doi.org/10.22028/D291-31037 |
ISSN: | 1560-2281 |
Datum des Eintrags: | 28-Mai-2020 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Systems Engineering |
Professur: | NT - Prof. Dr. Karsten König |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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014515_1.pdf | Translation of two-photon microscopy to the clinic | 5,25 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons