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Titel: A Photoactivatable α5β1‐Specific Integrin Ligand
VerfasserIn: Nair, Roshna V.
Farrukh, Aleeza
del Campo Bécares, Aránzazu
Sprache: Englisch
Titel: ChemBioChem : a European journal of chemical biology
Bandnummer: 19
Heft: 12
Startseite: 1280
Endseite: 1287
Verlag/Plattform: Wiley-VCH
Erscheinungsjahr: 2018
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The integrin α5β1 is overexpressed in colon, breast, ovarian, lung and brain tumours, and has been identified as key component in mechanosensing. In order to study how dynamic changes in α5β1 engagement affect cellular behaviour, photoactivatable derivatives of α5β1‐specific ligands are presented in this article. A photoremovable protecting group (PRPG) was introduced into the ligand structure at a relevant position for integrin recognition. The presence of the chromophore temporarily inhibited ligand bioactivity. Light exposure at a cell‐compatible dose efficiently cleaved the protecting group and restored functionality. The photoactive ligand had an azide end‐functional group for covalent immobilisation onto biomaterials by click chemistry. Selective cell responses (attachment, spreading, migration) to the activated ligand on the surface are achieved by controlled exposure to light, at similar levels to the native ligand. Spatial and temporal control of the cellular response is demonstrated, including the possibility of in situ activation. Photoactivatable integrin‐selective ligands in model microenvironments will allow the study of cellular behaviour in response to changes in the activation of individual integrins as consequence of dynamic variations in matrix composition.
DOI der Erstveröffentlichung: 10.1002/cbic.201800180
URL der Erstveröffentlichung: https://onlinelibrary.wiley.com/doi/full/10.1002/cbic.201800180
Link zu diesem Datensatz: hdl:20.500.11880/28563
http://dx.doi.org/10.22028/D291-29500
ISSN: 1439-7633
0044-8249
1439-4227
Datum des Eintrags: 13-Jan-2020
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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