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Titel: Linear Chains of HER2 Receptors Found in the Plasma Membrane Using Liquid-Phase Electron Microscopy
VerfasserIn: Parker, Kelly
Trampert, Patrick
Tinnemann, Verena
Peckys, Diana
Dahmen, Tim
de Jonge, Niels
Sprache: Englisch
Titel: Biophysical journal : BJ
Bandnummer: 115
Heft: 3
Startseite: 503
Endseite: 513
Verlag/Plattform: Cell Press
Erscheinungsjahr: 2018
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The spatial distribution of the human epidermal growth factor 2 (HER2) receptor in the plasma membrane of SKBR3 and HCC1954 breast cancer cells was studied. The receptor was labeled with quantum dot nanoparticles, and fixed whole cells were imaged in their native liquid state with environmental scanning electron microscopy using scanning transmission electron microscopy detection. The locations of individual HER2 positions were determined in a total plasma membrane area of 991 μm2 for several SKBR3 cells and 1062 μm2 for HCC1954 cells. Some of the HER2 receptors were arranged in a linear chain with interlabel distances of 40 ± 7 and 32 ± 10 nm in SKBR3 and HCC1954 cells, respectively. The finding was tested against randomly occurring linear chains of six or more positions, from which it was concluded that the experimental finding is significant and did not arise from random label distributions. Because the measured interlabel distance in the HER2 chains is similar to the 36-nm helix-repetition distance of actin filaments, it is proposed that a linking mechanism between HER2 and actin filaments leads to linearly aligned oligomers.
DOI der Erstveröffentlichung: 10.1016/j.bpj.2018.06.016
URL der Erstveröffentlichung: https://www.cell.com/biophysj/fulltext/S0006-3495(18)30719-7
Link zu diesem Datensatz: hdl:20.500.11880/28070
http://dx.doi.org/10.22028/D291-29250
ISSN: 1542-0086
0006-3495
Datum des Eintrags: 14-Okt-2019
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Physik
Professur: NT - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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