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Titel: Red Blood Cell Passage of Small Capillaries Is Associated with Transient Ca2+-mediated Adaptations
VerfasserIn: Danielczok, Jens G.
Terriac, Emmanuel
Hertz, Laura
Petkova-Kirova, Polina
Lautenschläger, Franziska
Laschke, Matthias W.
Kästner, Lars
Sprache: Englisch
Titel: Frontiers in physiology
Bandnummer: 8
Startseite: 1
Endseite: 11
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2017
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: When red blood cells (RBCs) pass constrictions or small capillaries they need to pass apertures falling well below their own cross section size. We used different means of mechanical stimulations (hypoosmotic swelling, local mechanical stimulation, passing through microfluidic constrictions) to observe cellular responses of human RBCs in terms of intracellular Ca2+-signaling by confocal microscopy of Fluo-4 loaded RBCs. We were able to confirm our in vitro results in a mouse dorsal skinfold chamber model showing a transiently increased intracellular Ca2+ when RBCs were passing through small capillaries in vivo. Furthermore, we performed the above-mentioned in vitro experiments as well as measurements of RBCs filterability under various pharmacological manipulations (GsMTx-4, TRAM-34) to explore the molecular mechanism of the Ca2+-signaling. Based on these experiments we conclude that mechanical stimulation of RBCs activates mechano-sensitive channels most likely Piezo1. This channel activity allows Ca2+ to enter the cell, leading to a transient activation of the Gardos-channel associated with K+, Cl-, and water loss, i.e., with a transient volume adaptation facilitating the passage of the RBCs through the constriction.
DOI der Erstveröffentlichung: 10.3389/fphys.2017.00979
URL der Erstveröffentlichung: https://www.frontiersin.org/articles/10.3389/fphys.2017.00979/full
Link zu diesem Datensatz: hdl:20.500.11880/27877
http://dx.doi.org/10.22028/D291-29004
ISSN: 1664-042X
Datum des Eintrags: 24-Sep-2019
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Physik
Professur: NT - Jun.-Prof. Dr. Franziska Lautenschläger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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